Epoxide hydrolase activity of Streptomyces strains.

  title={Epoxide hydrolase activity of Streptomyces strains.},
  author={F Zocher and M M Enzelberger and Uwe T Bornscheuer and Bernhard Hauer and Wolfgang Wohlleben and Rolf D. Schmid},
  journal={Journal of biotechnology},
  volume={77 2-3},
The discovery of epoxide hydrolases within a Streptomyces sp. strain collection is described. Screening was performed in 96 well microtiter plates using a modified 4-(p-nitrobenzyl)pyridine assay with styrene oxide, 1,2-epoxy-hexane or 3-phenyl ethylglycidate (3-PEG) as substrates. Out of 120 strains investigated, S. antibioticus Tü4, S. arenae Tü495 and S. fradiae Tü27 exhibited epoxide hydrolase activity. These strains were further investigated by performing laboratory-scale… CONTINUE READING