Epoxide electrophiles as activity-dependent cysteine protease profiling and discovery tools.

@article{Greenbaum2000EpoxideEA,
  title={Epoxide electrophiles as activity-dependent cysteine protease profiling and discovery tools.},
  author={Doron C. Greenbaum and Katalin F. Medzihradszky and Alma L. Burlingame and Matthew S Bogyo},
  journal={Chemistry \& biology},
  year={2000},
  volume={7 8},
  pages={
          569-81
        }
}
BACKGROUND Analysis of global changes in gene transcription and translation by systems-based genomics and proteomics approaches provides only indirect information about protein function. In many cases, enzymatic activity fails to correlate with transcription or translation levels. Therefore, a direct method for broadly determining activities of an entire class of enzymes on a genome-wide scale would be of great utility. RESULTS We have engineered chemical probes that can be used to broadly… 
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