Epitope mapping of nine monoclonal antibodies against osteocalcin: combinations into two-site assays affect both assay specificity and sample stability.

@article{Hellman1996EpitopeMO,
  title={Epitope mapping of nine monoclonal antibodies against osteocalcin: combinations into two-site assays affect both assay specificity and sample stability.},
  author={Jukka Hellman and Sanna M K{\"a}k{\"o}nen and M. T. Matikainen and Matti Karp and Timo L{\"o}vgren and H. Kalervo V{\"a}{\"a}n{\"a}nen and Kim Pettersson},
  journal={Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research},
  year={1996},
  volume={11 8},
  pages={1165-75}
}
Nine monoclonal antibodies (Mabs) were raised against human recombinant osteocalcin fusion protein (rGST-hOC) or bovine osteocalcin (bOC) and selected to develop two-site immunoassays for human osteocalcin (hOC). The detection system was based on the time-resolved measurement of the fluorescence of europium chelates conjugated to the tracer Mabs. Based on the ability of the Mabs to recognize different forms of hOC (carboxypeptidase Y-digested, alkylated hOC, thermally decarboxylated hOC… CONTINUE READING