Epitope distribution and immunochemical characterization of nucleolar phosphoprotein C23 using ten monoclonal antibodies

@article{Freeman1985EpitopeDA,
  title={Epitope distribution and immunochemical characterization of nucleolar phosphoprotein C23 using ten monoclonal antibodies},
  author={James W. Freeman and Amitava Chatterjee and Brenda E. Ross and Harris Busch},
  journal={Molecular and Cellular Biochemistry},
  year={1985},
  volume={68},
  pages={87-96}
}
Extractable nucleolar proteins from HeLa cells were used as a source of antigen to immunize mice for monoclonal antibody (MAb) production. Ten of the resulting MAbs shown to identify nucleolar phosphoprotein (110 kD/pI 5.5) were purified and used in immunochemical studies to further characterize protein C23. All ten MAbs showed nucleolar localization by… CONTINUE READING