Epitope analyses of pneumococcal surface protein A: a combination of two monoclonal antibodies detects 94% of clinical isolates.

@article{Kolberg2001EpitopeAO,
  title={Epitope analyses of pneumococcal surface protein A: a combination of two monoclonal antibodies detects 94% of clinical isolates.},
  author={Jan Kolberg and Audun Aase and T E Michaelsen and Gunnhild R\odal},
  journal={FEMS immunology and medical microbiology},
  year={2001},
  volume={31 3},
  pages={175-80}
}
Immunisation of BALB/c mice with seven heat-treated Norwegian clinical isolates of Streptococcus pneumoniae of different serotypes elicited mainly monoclonal antibodies (mAbs) to pneumococcal surface protein A (PspA). It was remarkable that the fusions resulted only in a few mAbs directed against other protein antigens. Dot blot analysis with 16 mAbs using clinical isolates representing 23 different capsular types and the uncapsulated reference strain R36A showed that some of the mAbs bound to… CONTINUE READING