Epithelial sodium channels (ENaC) are uniformly distributed on motile cilia in the oviduct and the respiratory airways

@article{Enuka2011EpithelialSC,
  title={Epithelial sodium channels (ENaC) are uniformly distributed on motile cilia in the oviduct and the respiratory airways},
  author={Yehoshua Enuka and Israel Hanukoglu and Oded Edelheit and Hananya Vaknine and Aaron Hanukoglu},
  journal={Histochemistry and Cell Biology},
  year={2011},
  volume={137},
  pages={339-353}
}
Epithelial sodium channels (ENaCs) are located on the apical surface of cells and funnel Na+ ions from the lumen into the cell. ENaC function also regulates extracellular fluid volume as water flows across membranes accompanying Na+ ions to maintain osmolarity. To examine the sites of expression and intracellular localization of ENaC, we generated polyclonal antibodies against the extracellular domain of human α-ENaC subunit that we expressed in E. coli. Three-dimensional (3D) confocal… Expand
Epithelial sodium channel (ENaC) in GtoPdb v.2021.2
The epithelial sodium channels (ENaC) are located on the apical membrane of epithelial cells in the kidney tubules, lung, respiratory tract, male and female reproductive tracts, sweat and salivaryExpand
Epithelial sodium channel (ENaC) family: Phylogeny, structure-function, tissue distribution, and associated inherited diseases.
TLDR
A comparative overview of the genotype-phenotype relationships in inherited diseases associated with ENaC mutations, including multisystem pseudohypoaldosteronism (PHA1B), Liddle syndrome, cystic fibrosis-like disease and essential hypertension is presented. Expand
Mapping the sites of localization of epithelial sodium channel (ENaC) and CFTR in segments of the mammalian epididymis
TLDR
To map the sites of localization of ion channels ENaC and CFTR along the length of the mouse and rat epididymis using confocal microscopic imaging, it is suggested that mutations in EN aC subunits may also be associated with male infertility. Expand
Epithelial sodium channel (ENaC) (version 2019.4) in the IUPHAR/BPS Guide to Pharmacology Database
The epithelial sodium channels (ENaC) are located on the apical membrane of epithelial cells in the distal kidney tubules, lung, respiratory tract, male and female reproductive tracts, sweat andExpand
Expression of epithelial sodium channel (ENaC) and CFTR in the human epidermis and epidermal appendages
TLDR
The results reveal that ENaC is expressed strongly in all epidermal layers except stratum corneum, and also in the sebaceous glands, eccrine glands, arrector pili smooth muscle cells, and intra-dermal adipocytes, and co-localized with F-actin. Expand
Progress in ciliary ion channel physiology
TLDR
This review focuses on the composition of putative ion channels found in both types of cilia and in the periciliary membrane and discusses their proposed functions. Expand
High‐resolution imaging of the actin cytoskeleton and epithelial sodium channel, CFTR, and aquaporin‐9 localization in the vas deferens
TLDR
The localization of all three channels on the apical surface of the columnar epithelial cells provides clear evidence that these channels are involved concurrently in the regulation of fluid and electrolyte balance in the lumen of the vas deferens. Expand
Cellular distribution and function of ion channels involved in transport processes in rat tracheal epithelium
TLDR
The data demonstrate that, in rat tracheal epithelium, Cl− secretion and Na+ absorption are routed through different cell types, and that ANO1 channels form the molecular basis of Ca2+‐dependent Cl‐ secretion in this tissue. Expand
Localization of epithelial sodium channel (ENaC) and CFTR in the germinal epithelium of the testis, Sertoli cells, and spermatozoa
TLDR
It is suggested that ENaC subunits in cytoplasmic pools in germ cells serve as the source of ENac subunits located along the tail of spermatozoa, and CFTR may have an independent role in gene regulation within the nuclei. Expand
Identification and classification of epithelial cells in nephron segments by actin cytoskeleton patterns
TLDR
This study highlights the power of high‐resolution confocal microscopy for identifying specific cell types using the simple probe of F‐actin‐binding phalloidin to identify whole kidney sections by confocal microscopic imaging of fluorescent phalloids. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 61 REFERENCES
Early expression of beta- and gamma-subunits of epithelial sodium channel during human airway development.
TLDR
Early expression of ENaC proteins in human fetal airways suggests that Na(+) absorption might begin significantly before birth, even if secretion is still dominant, as well as suggesting the distribution of its pore-forming alpha-, beta-, and gamma-subunits in the developing human lung. Expand
Overview of structure and function of mammalian cilia.
Cilia are membrane-bounded, centriole-derived projections from the cell surface that contain a microtubule cytoskeleton, the ciliary axoneme, surrounded by a ciliary membrane. Axonemes inExpand
Acute regulation of the epithelial sodium channel in airway epithelia by proteases and trafficking.
TLDR
Findings demonstrate that Na(+) absorption in the airway is acutely modulated by the coordinated trafficking of channels to the luminal surface and by the proteolytic activation of ENaC in response to ASL volume expansion. Expand
Motile Cilia of Human Airway Epithelia Are Chemosensory
TLDR
Airway epithelia contain a cell-autonomous system in which motile cilia both sense noxious substances entering airways and initiate a defensive mechanical mechanism to eliminate the offending compound. Expand
Soluble Mediators, Not Cilia, Determine Airway Surface Liquid Volume in Normal and Cystic Fibrosis Superficial Airway Epithelia
TLDR
It is found that maneuvers that mimic deposition of excess ASL onto the proximal airways, which occurs during mucociliary clearance and after glandular secretion, acutely stimulated Na+ absorption, suggesting that volume regulation was sensitive to changes in concentrations of soluble mediators in the ASL rather than alterations in ciliary beating. Expand
Differential expression and localization of CFTR and ENaC in mouse endometrium during pre‐implantation
TLDR
Differential expression and localization of CFTR and ENaC provide a molecular mechanism by which maximal fluid absorption can be achieved immediately prior to implantation, to ensure the immobilization of the blastocyst necessary for implantation. Expand
Identification of the roles of conserved charged residues in the extracellular domain of an epithelial sodium channel (ENaC) subunit by alanine mutagenesis.
TLDR
It is established that while some conserved charged residues are part of the structure responsible for Na(+) self-inhibition, most are essential for transport to the oocyte cell surface. Expand
Cell-specific expression of epithelial sodium channel alpha, beta, and gamma subunits in aldosterone-responsive epithelia from the rat: localization by in situ hybridization and immunocytochemistry
TLDR
The data indicate that alpha, beta, and gamma subunit mRNAs and proteins are co-expressed in the distal nephron (excepting IMCD), a localization that correlates with the previously described physiological expression of amiloride-sensitive electrogenic sodium transport. Expand
Early death due to defective neonatal lung liquid clearance in alpha-ENaC-deficient mice.
TLDR
It is shown that ENaC plays a critical role in the adaptation of the newborn lung to air breathing and electrogenic Na+ transport was abolished in airway epithelia from alpha-ENaC(-/-) mice. Expand
Regulation of the depth and composition of airway surface liquid
TLDR
In airway diseases, the balance is shifted from water secretion to mucus secretion, and in bronchitis and asthma this is due mainly to conversion of gland serous to mucous cells. Expand
...
1
2
3
4
5
...