Epigenetics: Writing the histone code


Crabtree sees more potential applications of the chromatin in vivo assay. “The technique allows the study of the mechanistic underpinnings of reprogramming,” he notes. For Crabtree, one of the big lessons of this work lay not so much in what they discovered but in what they did not see. “It allows us to predict where the holes are,” he says. When they first developed the model for H3K9me3 spreading, Crabtree recalls being pleased with the result; however, it soon became clear that during the first few hours of HP1 recruitment, something was going on that they did not understand. HP1 is recruited rapidly, but the first H3K9 methylation mark is much slower to appear. Something seems to occupy this locus that first needs to be removed. Crabtree calls this factor the ‘nonvisible part’ of their assay and has some ideas, such as using RNA interference or small molecule screens, for figuring out what is happening during the first few hours of chromatin silencing. Now that the histone code can be actively (re)written, much waits to be explored. nicole rusk

DOI: 10.1038/nmeth.2116

Cite this paper

@article{Rusk2012EpigeneticsWT, title={Epigenetics: Writing the histone code}, author={Nicole Rusk}, journal={Nature Methods}, year={2012}, volume={9}, pages={777-777} }