Epigenetic landscapes reveal transcription factors regulating CD8+ T cell differentiation

Abstract

Dynamic changes in the expression of transcription factors (TFs) can influence specification of distinct CD8+ T cell fates, but the observation of equivalent expression of TF among differentiallyfated precursor cells suggests additional underlying mechanisms. Here, we profiled genome-wide histone modifications, open chromatin and gene expression of naive, terminal-effector, memoryprecursor and memory CD8+ T cell populations induced during the in vivo response to bacterial Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms Correspondence should be addressed to A.W.G. (agoldrath@ucsd.edu) and W.W. (wei-wang@ucsd.edu). 10Present address: Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil 11These authors contributed equally to this work AUTHOR CONTRIBUTIONS B.Y. designed and performed experiments, analyzed the data and wrote the paper; K.Z. performed computational analysis and wrote the paper; B.Y., J.J.M., R.C. and C.T. performed shRNA knockdown; R.M.P and J.P.S-B. provided ATAC-seq datasets for polyclonal CD8+ T cell populations. M.E.P. and S.C. provided reagents, advice for the design of experiments and analysis of experiments and assisted in writing the paper; J.T.C. provided advice and assisted in writing the paper. W.W. supervised the computational analysis and wrote the paper; and A.W.G. supervised the project, designed the experiments, analyzed the data, and wrote the paper. COMPETING FINANCIAL INTERESTS STATEMENT The authors declare no competing financial interests. ACCESSION CODES GEO: ATAC-seq and ChIP-seq: GSE89036, microarray: GSE89037 HHS Public Access Author manuscript Nat Immunol. Author manuscript; available in PMC 2017 September 13. Published in final edited form as: Nat Immunol. 2017 May ; 18(5): 573–582. doi:10.1038/ni.3706. A uhor M anscript

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@inproceedings{Yu2017EpigeneticLR, title={Epigenetic landscapes reveal transcription factors regulating CD8+ T cell differentiation}, author={Bingfei Yu and Kai Zhang and J. J. Milner and Clara Toma and Runqiang Chen and James P. Scott-Browne and Renata Magrino Pereira and Shane Crotty and John T. Chang and Matthew E. Pipkin and Wei Wang and Ananda W. Goldrath}, year={2017} }