Epigenetic down regulation of nerve growth factor during alcohol withdrawal

  title={Epigenetic down regulation of nerve growth factor during alcohol withdrawal},
  author={Annemarie Heberlein and Marc An Muschler and Helge Frieling and M Behr and Christian K. Eberlein and Julia Wilhelm and Michael Gr{\"o}schl and Johannes Kornhuber and Stefan Bleich and Thomas Hillemacher},
  journal={Addiction Biology},
We investigated the Cytosin‐phosphatidyl‐Guanin (CpG) island promoter methylation (mean and methylation of individual CpG‐sites) of the nerve growth factor (NGF) gene in the blood of alcohol‐dependent patients (57 male patients) during withdrawal (days 1, 7 and 14). Methylation and NGF serum levels did not change significantly from days 1–7. From days 7–14, mean methylation increased (F = 30.55, P < 0.001), whereas the NGF serum levels decreased significantly (days 7–14: F = 17.95, P < 0.001… 
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Serum Brain-Derived Neurotrophic Factor and Nerve Growth Factor Concentrations Change after Alcohol Withdrawal: Preliminary Data of a Case-Control Comparison
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Alcohol intake during prenatal life affects neuroimmune mediators and brain neurogenesis.
  • L. Aloe
  • Medicine
    Annali dell'Istituto superiore di sanita
  • 2006
The result of the study indicates that NGF synthesis and the functional activity of NGF-target cells localized in the brain are markedly influenced by ethanol intake.
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Recent discoveries involving novel and diverse biological activities of NGF are discussed, suggesting that alterations in normal mast cell behaviours can provoke maladaptive neuroimmune tissue responses whose consequences could have profound implications in inflammatory disease states.
GM1 and nerve growth factor modulate mitochondrial membrane potential and neurofilament light mRNA expression in cultured dorsal root ganglion and spinal cord neurons during excitotoxic glutamate exposure
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The conventional view that DNA methylation functions predominantly to irreversibly silence transcription is being challenged and not only is promoter methylation often highly dynamic during development, but many organisms also seem to targetDNA methylation specifically to the bodies of active genes.
Quantitative DNA methylation analysis based on four-dye trace data from direct sequencing of PCR amplificates
A novel quantitative methylation analysis algorithm and workflow based on direct DNA sequencing of PCR products from bisulfite-treated DNA with high-throughput sequencing machines is developed and the applicability is proven by identifying CpGs that are differentially methylated in real tissue samples.
Society for the study of addiction.