Epigenetic control of transgene expression and imprinting by genotype-specific modifiers.

Abstract

Expression and DNA methylation of the transgene locus TKZ751 are controlled by genotype-specific modifier genes. The DBA/2 and 129 genetic backgrounds enhanced expression, while the BALB/c background suppressed expression, but only following maternal inheritance of the BALB/c modifier. Epigenetic modification of the transgene locus was cumulative over successive generations, which in BALB/c mice resulted in an irreversible methylation after three consecutive germline passages. Therefore, at the TKZ751 locus the germline fails to reverse previously acquired epigenetic modifications, a process that is usually essential to restore the genomic totipotency. Hence the genotype-specific modifier genes regulate penetrance and expressivity as well as parental imprinting of the TKZ751 locus through epigenetic modification.

Statistics

0100200'93'96'99'02'05'08'11'14'17
Citations per Year

1,770 Citations

Semantic Scholar estimates that this publication has 1,770 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Allen1990EpigeneticCO, title={Epigenetic control of transgene expression and imprinting by genotype-specific modifiers.}, author={Nicholas D. Allen and Megan L Norris and M Azim Surani}, journal={Cell}, year={1990}, volume={61 5}, pages={853-61} }