Epigenetic characterization of the early embryo with a chromatin immunoprecipitation protocol applicable to small cell populations

@article{Oneill2006EpigeneticCO,
  title={Epigenetic characterization of the early embryo with a chromatin immunoprecipitation protocol applicable to small cell populations},
  author={L. O'neill and M. VerMilyea and B. Turner},
  journal={Nature Genetics},
  year={2006},
  volume={38},
  pages={835-841}
}
  • L. O'neill, M. VerMilyea, B. Turner
  • Published 2006
  • Medicine, Biology
  • Nature Genetics
  • Chromatin immunoprecipitation (ChIP) defines the genomic distribution of proteins and their modifications but is limited by the cell numbers required (ideally >107). Here we describe a protocol that uses carrier chromatin and PCR, 'carrier' ChIP (CChIP), to permit analysis of as few as 100 cells. We assayed histone modifications at key regulator genes (such as Nanog, Pou5f1 (also known as Oct4) and Cdx2) by CChIP in mouse embryonic stem (ES) cells and in inner cell mass (ICM) and trophectoderm… CONTINUE READING
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