Epidermal growth factor-induced contraction regulates paxillin phosphorylation to temporally separate traction generation from de-adhesion.

@article{Schneider2009EpidermalGF,
  title={Epidermal growth factor-induced contraction regulates paxillin phosphorylation to temporally separate traction generation from de-adhesion.},
  author={Ian C Schneider and Cristen K Hays and Clare M. Waterman},
  journal={Molecular biology of the cell},
  year={2009},
  volume={20 13},
  pages={
          3155-67
        }
}
Directed cell migration is mediated by cycles of protrusion, adhesion, traction generation on the extracellular matrix and retraction. However, how the events after protrusion are timed, and what dictates their temporal order is completely unknown. We used acute epidermal growth factor (EGF) stimulation of epidermal keratinocytes to initiate the cell migration cycle to study the mechanism of the timing of adhesion, traction generation, and de-adhesion. Using microscopic and biochemical assays… CONTINUE READING