Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes

  title={Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes},
  author={James G. Rheinwald and Howard Green},
The culture lifetime of epidermal cells of newborn humans is increased from 50 to 150 generations by adding to the medium epidermal growth factor, a polypeptide mitogen. EGF seems to delay senescence of the cells by maintaining them in a state further removed from terminal differentiation. This effect is revealed by a greater ability of the cells to survive subculture and initiate new colonies, but not necessarily by an increased growth rate. 
An endocrine approach to the control of epidermal growth: serum-free cultivation of human keratinocytes.
It is suggested that control of keratinocyte growth depends in large part on endocrine stimulation by other body organs, including the brain, when grown in a hormone-supplemented medium.
Cultivation of keratinocytes derived from epidermal explants of sheep skin and the roles of growth factors in the regulation of proliferation
The detection of basic-FGF immunoreactivity in Western immunoblats of extracts of fresh tissues suggests a role for this factor in autocrine or paracrine growth regulation of skin cell populations in vivo.
Growth of cultured human epidermal cells into multiple epithelia suitable for grafting.
It will be shown here that large amounts of cultured epithelium can be generated from a small piece of epidermis in a short time.
In Vitro Expanded Human Keratinocytes: Clinical and Biological Experiences
Until recently it seemed impossible to culture human keratinocytes. In 1975, the use of a feeder layer of irradiated mouse-fibroblasts in the culture of the epithelial cells opened the way to the
Culture techniques for human keratinocytes.
Control of growth and differentiation in vitro of human keratinocytes cultured in serum-free medium.
The data suggest that the control of proliferation and differentiation in keratinocytes may take place by unique mechanisms and provide an excellent system for the study of nutritional disorders that affect human epidermal cells.
Autonomous growth of BALB/MK keratinocytes transfected with a retroviral vector carrying the human epidermal growth factor gene
Keratinocyte transfection with EGF cDNA provides a time- and cost-efficient means of culturing keratinocytes and yields cells that may be useful for skin grafting.
Long-term cultivation of canine keratinocytes.
The use of cultured canine keratinocytes should provide a suitable model for comparative in vitro studies of the pathogenesis of dermatologic diseases.


Density dependent proliferation of human glia cells stimulated by epidermal growth factor.
  • B. Westermark
  • Biology, Medicine
    Biochemical and biophysical research communications
  • 1976
Effect of foetal calf serum and epidermal growth factor on DNA synthesis in explants of chick embryo epidermis
It is reported that EGF considerably enhances precursor incorporation into DNA of chick embryo epidermal explants and that the responsiveness of the tissue to the hormone is increased by foetal calf serum.
Serum Albumin Supplemented Medium for Long Term Cultivation of Mammalian Fibroblast Strains.∗
  • G. Todaro, H. Green
  • Chemistry, Medicine
    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine
  • 1964
Summary Serially transferred hamster or human fibroblasts maintained on synthetic medium containing 10% calf serum could be carried through about 10 and about 50-70 cell generations respectively,
Epidermal growth factor: receptors in human fibroblasts and modulation of action by cholera toxin.
Observations indicate that fibroblasts possess receptors for EGF by biological and physicochemical criteria, and suggest that a similar if not identical peptide may be amongst those factors in sera which stimulate cell growth.
Effect of fibroblast growth factor on the division and fusion of bovine myoblasts
Growth hormone, insulin and testosterone did not have significant effects on DNA synthesis in bovine myoblasts when compared to the FGF and FGF did not stimulate the proliferation of chick embryo myoblast, indicating that it is not active in all vertebrate species.