Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes

@article{Rheinwald1977EpidermalGF,
  title={Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes},
  author={James G. Rheinwald and Howard Green},
  journal={Nature},
  year={1977},
  volume={265},
  pages={421-424}
}
The culture lifetime of epidermal cells of newborn humans is increased from 50 to 150 generations by adding to the medium epidermal growth factor, a polypeptide mitogen. EGF seems to delay senescence of the cells by maintaining them in a state further removed from terminal differentiation. This effect is revealed by a greater ability of the cells to survive subculture and initiate new colonies, but not necessarily by an increased growth rate. 
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