Epidemic population structure of extraintestinal pathogenic Escherichia coli determined by single nucleotide polymorphism pyrosequencing.

@article{FernndezRomero2011EpidemicPS,
  title={Epidemic population structure of extraintestinal pathogenic Escherichia coli determined by single nucleotide polymorphism pyrosequencing.},
  author={Natalia Fern{\'a}ndez-Romero and Mar{\'i}a Pilar Romero-G{\'o}mez and Mar{\'i}a Rosa G{\'o}mez-Gil and Jes{\'u}s Mingorance},
  journal={Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases},
  year={2011},
  volume={11 7},
  pages={
          1655-63
        }
}
We have developed an MLST-based scheme for typing Escherichia coli isolates using pyrosequencing of single nucleotide polymorphic positions (SNP). The SNP sequences are converted into allelic patterns and analyzed using the same approach used for MLST analyses. We have tested the method in two unselected collections of clinical isolates of E. coli obtained from blood and urine cultures. The two collections had a similar structure, 25% of the profiles (representing 68% of the isolates) were… CONTINUE READING

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