Eph Receptors and Ligands Comprise Two Major Specificity Subclasses and Are Reciprocally Compartmentalized during Embryogenesis

@article{Gale1996EphRA,
  title={Eph Receptors and Ligands Comprise Two Major Specificity Subclasses and Are Reciprocally Compartmentalized during Embryogenesis},
  author={Nicholas W. Gale and Sacha J. Holland and David M. Valenzuela and Ann M. Flenniken and Li Pan and Terrence E Ryan and Mark Henkemeyer and Klaus Strebhardt and Hisamaru Hirai and David G. Wilkinson and Tony Pawson and Samuel Davis and George D. Yancopoulos},
  journal={Neuron},
  year={1996},
  volume={17},
  pages={9-19}
}

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The identification and characterization of the third, and most divergent, member of the transmembrane group of Eph ligands, which is term Elk-L3 (Elk-related receptor ligand number 3), which is notable for its remarkably restricted and prominent expression in the floor plate and roof plate of the developing neural tube and its rhombomere-specificexpression in the developing hindbrain.
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The recently discovered family of RTKs termed Eph, which currently consists of seven distinct members, eph, eck, elk, cek5, mek4/cek 4/hek, sek and hek2, all of whose cDNAs have been fully sequenced, appears to be the largest known family ofRTKs.
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A new member of the Eph ligand family is described, designated ELF-2, which shows closest homology to the other known transmembrane ligand in the family, ELK-L/LERK-2/Cek5-L, with 57% identity in the extracellular domain and striking homology in the cytoplasmic domain.
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The identification and characterization of a novel member of the Eph receptor family is described, which is termed Ehk-3 for Eph Homologous Kinase 3, which displays all the major structural features shared by other members of theEph family, including a cysteine-rich region and tandem fibronectin type-III domains in its extracellular portion.
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Using a soluble form of hek consisting of the extracellular region of the receptor fused to the Fc domain of human IgG1 and an expression cloning strategy, two different but related cDNAs from the human T-lymphoma line HSB-2 that encode ligands for hek are isolated.
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