Enzymic synthesis of lignin precursors. Purification and properties of UDP glucose: coniferyl-alcohol glucosyltransferase from cambial sap of spruce (Picea abies L.).

@article{Schmid1982EnzymicSO,
  title={Enzymic synthesis of lignin precursors. Purification and properties of UDP glucose: coniferyl-alcohol glucosyltransferase from cambial sap of spruce (Picea abies L.).},
  author={G. Cardinet Schmid and Hans Theodor Grisebach},
  journal={European journal of biochemistry},
  year={1982},
  volume={123 2},
  pages={
          363-70
        }
}
UDPglucose:coniferyl-alcohol glucosyltransferase was isolated from cambial sap of spruce (Picea abies). An apparently homogeneous enzyme was obtained by a seven-step procedure including dye-ligand chromatography. The enzyme has an Mr of about 50 000 and consists of one polypeptide chain. Transferase activity is not influenced by metal ions. The enzyme shows a pronounced substrate specificity towards UDPglucose and coniferyl alcohol with Km values of respectively 220 microM and 250 microM. The… CONTINUE READING
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