Enzymic reconstruction of glycosaminoglycan oligosaccharide chains using the transglycosylation reaction of bovine testicular hyaluronidase.

@article{Saitoh1995EnzymicRO,
  title={Enzymic reconstruction of glycosaminoglycan oligosaccharide chains using the transglycosylation reaction of bovine testicular hyaluronidase.},
  author={Hiroshi Saitoh and Keiichi Takagaki and Mitsuo Majima and Tsutomu Nakamura and Atsushi Matsuki and Masanori Kasai and Hiroshi Narita and Masahiko Endo},
  journal={The Journal of biological chemistry},
  year={1995},
  volume={270 8},
  pages={
          3741-7
        }
}
The reconstruction of glycosaminoglycan chains using the transglycosylation reaction of testicular hyaluronidase was investigated. First, the optimal conditions for the transglycosylation reaction catalyzed by the enzyme were determined by incubation with the enzyme, using hyaluronic acid (M(r) = 800,000) as a donor and pyridylaminated hyaluronic acid hexasaccharide having glucuronic acid at the nonreducing terminal as an acceptor. The carbohydrate chains as reaction products were determined by… CONTINUE READING
BETA

Citations

Publications citing this paper.
SHOWING 1-10 OF 18 CITATIONS

Similar Papers

Loading similar papers…