Enzymic and non-enzymic liberation of tulipalin A (α-methylene butyrolactone) in extracts of tulip

  title={Enzymic and non-enzymic liberation of tulipalin A ($\alpha$-methylene butyrolactone) in extracts of tulip},
  author={J. C. M. Beijersbergen and Catharina B. G. Lemmers},
  journal={Physiologial Plant Pathology},
Purification and Characterization of a Tuliposide-Converting Enzyme from Bulbs of Tulipa gesneriana
An enzyme that catalyzes the stoichiometric conversion of 6-tuliposide into tulipalin was purified and characterized from bulbs of Tulipa gesneriana and had maximum activity and stability at neutral pH and moderate temperature.
Antibacterial effects of S-(-)-tulipalin B isolated from Spiraea thunbergii Sieb. on Escherichia coli, a major food borne pathogenic microorganism
The crude ethanol extract of the leaves of Spiraea thunbergii Sieb. (Rosaceae) showed antibacterial activity against a food borne pathogen, Escherichia coli. Bioactivity-guided separation led to the
Anther-specific Production of Antimicrobial Tuliposide B in Tulips
The results suggest that a novel defense strategy evolved in tulips to protect pollens from bacterial pollution in the reproductive process by producing an anther-specific 6-tuliposide B.
Involvement of strictosidine as a defensive chemical inCatharanthus roseus
Strictosidine and its deglucosylation product, specifically formed by the enzyme strictosidine glucosidase, were shown to be active against several microorganisms.
Molecular identification of tuliposide B-converting enzyme: a lactone-forming carboxylesterase from the pollen of tulip.
Results indicate that mature TgTCEB1 is specifically localized in plastids of pollen grains, but not in the intracellular spaces of pollen grain, as previously reported.
A Novel Lactone-Forming Carboxylesterase: Molecular Identification of a Tuliposide A-Converting Enzyme in Tulip1[W]
To the knowledge, TgTCEA is the first identified member of the lactone-forming carboxylesterases, specifically catalyzing intramolecular transesterification in the tulip defensive strategy.
One-Step Enzymatic Synthesis of 1-Tuliposide A Using Tuliposide-Converting Enzyme
By using the TCEs, this work established a facile enzymatic process for 1-PosA synthesis from the naturally occurring 1,6-diacyl-glucose type of Pos (PosD and PosF), and discovered that TCEA and TCEB react preferentially with PosD andPosF, respectively, to form 1- PosA and the corresponding Pa derived from the 6-acyl group.
Environmentally benign process for the preparation of antimicrobial α-methylene-β-hydroxy-γ-butyrolactone (tulipalin B) from tulip biomass
An environmentally benign and facile process for the preparation of PaB using tulip biomass materials based on one-step enzyme reaction catalyzed by TCE without the use of petroleum-derived solvents is described.
Molecular diversity of tuliposide B-converting enzyme in tulip (Tulipa gesneriana): identification of the root-specific isozyme
Tissue-specific isozymes are involved in the conversion of 6-tuliposide B, a major secondary metabolite in tulip, to antibacterial tulipalin B, which encodes a TCEB.


Über Glykoside mit lacton-bildendem Aglykon, II. Über die Struktur der antibiotisch aktiven Substanzen der Tulpe (Tulipa gesneriana L.)
Aus Tulipa gesneriana L. wurden zwei antibiotisch aktive Glucoside, 1-Tuliposid A (14a) und 1-Tuliposid B (15a), isoliert, die Acyl-β-D-glucopyranoside der γ-Hydroxy-α-methylen-buttersaure bzw.