Enzymic analysis of the crabtree effect in glucose-limited chemostat cultures of Saccharomyces cerevisiae.

@article{Postma1989EnzymicAO,
  title={Enzymic analysis of the crabtree effect in glucose-limited chemostat cultures of Saccharomyces cerevisiae.},
  author={E. J. Postma and Cornelis Verduyn and W. Alexander Scheffers and Johannes P van Dijken},
  journal={Applied and environmental microbiology},
  year={1989},
  volume={55 2},
  pages={468-77}
}
The physiology of Saccharomyces cerevisiae CBS 8066 was studied in glucose-limited chemostat cultures. Below a dilution rate of 0.30 h-1 glucose was completely respired, and biomass and CO2 were the only products formed. Above this dilution rate acetate and pyruvate appeared in the culture fluid, accompanied by disproportional increases in the rates of oxygen consumption and carbon dioxide production. This enhanced respiratory activity was accompanied by a drop in cell yield from 0.50 to 0.47 g… CONTINUE READING
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