Enzymes of UDP‐GlcNAc biosynthesis in yeast

  title={Enzymes of UDP‐GlcNAc biosynthesis in yeast},
  author={Sławomir Milewski and Iwona Gabriel and Jarosław Olchowy},
D‐Glucosamine is an important building block of major structural components of the fungal cell wall, namely chitin, chitosan and mannoproteins. Other amino sugars, such as D‐mannosamine and D‐galactosamine, relatively abundant in higher eukaryotes, rarely occur in fungal cells and are actually absent from yeast and yeast‐like fungi. The glucosamine‐containing sugar nucleotide UDP‐GlcNAc is synthesized in yeast cells in a four‐step cytoplasmic pathway. This article provides a comprehensive… 
N-Acetylglucosamine Regulates Morphogenesis and Virulence Pathways in Fungi
The recent advances in understanding the role of GlcNAc signaling pathways in regulating C. albicans morphogenesis and virulence are described.
Acetamido Sugar Biosynthesis in the Euryarchaea
UDP-GlcNAc and UDP-ManNAcA biosynthesis evolved early in the euryarchaeal lineage, because most of their genomes contain orthologs of the five genes characterized here.
Targeting a critical step in fungal hexosamine biosynthesis
Genetic, structural, and chemical evidence that Gna1 is an antifungal target in A. fumigatus is provided and a small heterocyclic scaffold is discovered that binds proximal to the GNA1 active site and can be optimized to a selective submicromolar binder.
Genetic and structural validation of Aspergillus fumigatus UDP-N-acetylglucosamine pyrophosphorylase as an antifungal target
It is demonstrated that the fungal pathogen Aspergillus fumigatus possesses an active UAP (AfUAP1) that shows selectivity for GlcNAc‐1P as the phosphosugar substrate, which could represent a novel antifungal target and will assist the future discovery of small molecule inhibitors against this enzyme.
Characterization of N‐Acetylglucosamine Biosynthesis in Pneumocystis species. A New Potential Target for Therapy
Evidence for a uridine diphospho (UDP)‐GlcNAc pathway in Pneumocystis species and the presence of at least four proteins implicated in the Saccharomyces cerevisiae UDP‐GlCNAc biosynthetic pathway are provided.
N-acetylglucosamine Regulates Virulence Properties in Microbial Pathogens
The role of these new GlcNAc signaling pathways in the regulation of virulence factors will be the focus of this review.
The N‐acetylglucosamine catabolic gene cluster in Trichoderma reesei is controlled by the Ndt80‐like transcription factor RON1
Functional analysis in Trichoderma reesei showed that the transcription factor RON1 is a key activator of the GlcNAc gene cluster and essential for Glc NAc catabolism, and an evolutionary analysis of Ndt80‐like proteins in Ascomycota is presented.
N-Acetylglucosamine Functions in Cell Signaling
The emerging roles of GlcNAc as an activator and mediator of cellular signaling in fungi, animals, and bacteria will be the focus of this paper.


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    FASEB journal : official publication of the Federation of American Societies for Experimental Biology
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Yeast mutants are useful to identify biosynthetic intermediates, to establish whether a given enzyme is essential for viability, and to determine how cellular functions are affected when glycosylation is perturbed.
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The pattern of induction for these enzymes was the same under conditions of germ-tube formation and where yeast cells metabolized GlcNAc with no change in morphology, indicating that these enzymes are not control points in the dimorphic development of C. albicans.
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The antibiotic tetaine inhibits in Candida albicans the biosynthesis of two important cell wall constituents, chitin and mannoprotein, and in the presence of tetaine, probably a modified mannobrotein, lacking a branched polymannan, is synthesized.
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  • Biology, Chemistry
    Biochimica et biophysica acta
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The Candida albicans GFA1 gene encoding glucosamine-6-phosphate synthase, an enzyme of cell wall biosynthesis pathway in fungi and bacteria, recently an object of interest as a target for the
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