Enzymes involved in crotonate metabolism in Syntrophomonas wolfei

  title={Enzymes involved in crotonate metabolism in Syntrophomonas wolfei},
  author={Michael J. McInerney and Neil Q. Wofford},
  journal={Archives of Microbiology},
Cell-free extracts of Syntrophomonas wolfei subsp. wolfei grown with crotonate in pure culture or in coculture with Methanospirillum hungatei contained crotonyl-coenzyme A (CoA): acetate CoA-transferase activity. This activity was not detected in cell-free extracts from the butyrate-grown coculture which suggests that the long lag times observed before S. wolfei grew with crotonate were initially due to the inability to activate crotonate. Cell-free extracts of S. wolfei grown in pure culture… 

A Proteomic View at the Biochemistry of Syntrophic Butyrate Oxidation in Syntrophomonas wolfei

An electron flow scheme for syntrophic butyrate oxidation in S. wolfei is depicted, allowing to depict an ATP hydrolysis-driven proton-motive force across the cytoplasmatic membrane that would provide the energy input for the electron potential shift necessary for formate formation.

Localization of the enzymes involved in H2 and formate metabolism inSyntrophospora bryantii

Cell-free extracts of crotonate-grown cells of the syntrophic butyrate-oxidizing bacteriumSyntrophospora bryantii suggest that during syntrophicbutyrate oxidation H2 is formed intracellularly while formate is formed at the outside of the cell.

Evidence of reversed electron transport in syntrophic butyrate or benzoate oxidation by Syntrophomonas wolfei and Syntrophus buswellii

The results indicate that a proton-potential-driven process is involved in hydrogen release from butyrate or benzoate oxidation, and localization of enzymes involved inbutyrate oxidation by S. wolfei and S. buswellii revealed that ATP synthase, hydrogenase, and butyryl-CoA dehydrogenase were at least partially membrane-associated.

Activation and degradation of benzoate, 3-phenylpropionate and crotonate bySyntrophus buswellii strain GA. Evidence for electron-transport phosphorylation during crotonate respiration

A strictly anaerobic, benzoate-degrading bacterium,Syntrophus buswellii strain GA, was able to degrade benzoate or 3-phenylpropionate to acetate, CO2 and H2 if the hydrogen partial pressure was

The genome of Syntrophomonas wolfei: new insights into syntrophic metabolism and biohydrogen production.

The genome sequence of S. wolfei reveals several core reactions that may be characteristic of syntrophic fatty acid metabolism and illustrates how biological systems produce hydrogen from thermodynamically difficult reactions.

Syntrophic Degradation of Cadaverine by a Defined Methanogenic Coculture

Comparative 16S rRNA gene analysis indicated that the fermenting part of the coculture belongs to the subphylum Firmicutes but that this part is distant from any described genus.

Dynamic acylome reveals metabolite driven modifications in Syntrophomonas wolfei

These findings link protein acylation by RACS to shifts in cellular metabolism, and identify types of acylations comprehensively, two of which have not been reported in any system previously.

The Family Syntrophomonadaceae

The family Syntrophomonadaceae comprises the genera syntrophomonas, Pelospora, Syntrophothermus, and Thermosyntropha, which are Gram-positive with low DNA content, but in most cases the murein layer is thin and an outer membrane appears, resembling the cell wall architecture of Gram-negative bacteria.

Syntrophism among Prokaryotes

Want to understand how microorganisms act in natural systems requires the realization that microorganisms don’t usually occur as pure cultures out there, but that every single cell has to cooperate or compete with other microor macroorganisms.



Preparation of cell-free extracts and the enzymes involved in fatty acid metabolism in Syntrophomonas wolfei

Syntrophomonas wolfei is an anaerobic fatty acid degrader that can only be grown in coculture with H2-using bacteria such as Methanospirillum hungatei and cell extracts obtained with this method had high specific activities, indicating that fatty acid activation occurred by transfer of CoA from acetyl-CoA.

Effects of Organic Acid Anions on the Growth and Metabolism of Syntrophomonas wolfei in Pure Culture and in Defined Consortia

The results showed that the metabolism of S. wolfei was inhibited by high levels of organic acid anions, and the activity of acetate-using methanogens is important for the syntrophic degradation of fatty acids when high levelsof acetate are present.

Intermediary Metabolism in Clostridium acetobutylicum: Levels of Enzymes Involved in the Formation of Acetate and Butyrate

The results suggest that the uptake of acetate and butyrate during solvent formation can not proceed via a complete reversal of the phosphate transferase and kinase reactions.

The coenzyme A transphorase system in Clostridium kluyveri.

  • E. Stadtman
  • Chemistry, Biology
    The Journal of biological chemistry
  • 1953

Electron transport in Azotobacter vinelandii.

Syntrophomonas wolfei gen. nov. sp. nov., an Anaerobic, Syntrophic, Fatty Acid-Oxidizing Bacterium

Three strains of the bacterium were characterized and are described as a new genus and species, Syntrophomonas wolfei, which contains poly-beta-hydroxybutyrate, and the presence of muramic acid, inhibition of growth by penicillin, and increased sensitivity of the cells to lysis after treatment with lysozyme indicate that peptidoglycan is present in the cell wall.


A partially purified butyrokinase from Clostridium tetanomorphum has been found to phosphorylate valerate, butyrate, isobutyrate and propionate, and the divalent cation requirement is satisfied with magnesium.

Kinetics and catalytic properties of coenzyme A transferase from Peptostreptococcus elsdenii

Coenzyme A (CoA) transferase from Peptostreptococcus elsdenii was purified to homogeneity, and some of its physical and catalytic properties were determined. The native enzyme has a molecular weight

Isolation and characterization of an anaerobic syntrophic benzoate-degrading bacterium from sewage sludge

An anaerobic, motile, gram-negative, rod-shaped bacterium is described which degrades benzoate in coculture with an H2-utilizing organism and in the absence of exogenous electron acceptors such as

Cytochrome spectrum of an obligate anaerobe, Eubacterium lentum

An obligately anaerobic bacterium, Eubacterium lentum, was shown to contain cytochromes a, b, and c and a carbon monoxide-binding pigment and quantitation of protoheme IX and heme c.