Enzyme-substrate interaction in lipid monolayers. II. Binding and activity of lipase in relation to enzyme and substrate concentration and to other factors.


With the limited stirring procedure used in the present work, substrate and enzyme together form a segregated and well-defined system on the surface. The lipase molecules responsible for the lipolysis are only those that are adsorbed on the glyceride monolayer. After a study of the stirring procedure, two series of systematic experiments were done: a) the… (More)

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