Enzyme purification using temperature-induced phase formation.

@article{Harris1991EnzymePU,
  title={Enzyme purification using temperature-induced phase formation.},
  author={P A Harris and Gunnar Karlstr{\"o}m and Folke Tjerneld},
  journal={Bioseparation},
  year={1991},
  volume={2 4},
  pages={237-46}
}
A new type of aqueous two-phase system composed of an ethylene oxide and propylene oxide random co-polymer, UCON 50-HB-5100, as the upper phase polymer and either dextran or hydroxypropyl starch as the lower phase polymer has been characterized and used to purify 3-phosphoglycerate kinase (EC 2.7.2.3) and hexokinase (EC 2.7.1.1) from bakers' yeast. The UCON 50-HB-5100 polymer has a cloud point of 55 degrees C at which temperature it phase separates from water. This cloud point can be lowered to… CONTINUE READING