Enzyme mimicry by the antiidiotypic antibody approach.

@article{Kolesnikov2000EnzymeMB,
  title={Enzyme mimicry by the antiidiotypic antibody approach.},
  author={Alexander V. Kolesnikov and Arina V Kozyr and E. S. Alexandrova and F Koralewski and Alexander Victorovich Demin and Michail I. Titov and Bérangère Avalle and Alfonso Tramontano and Sudhir Paul and D. Thomas and Alexander G. Gabibov and Alain Friboulet},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2000},
  volume={97 25},
  pages={
          13526-31
        }
}
  • A. Kolesnikov, A. Kozyr, +9 authors A. Friboulet
  • Published 5 December 2000
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
The concept of "internal image" of antiidiotypic antibodies has provided the basis for eliciting catalytic antibodies. A monoclonal IgM 9A8 that was obtained as an antiidiotype to AE-2 mAb, a known inhibitor of acetylcholinesterase, displayed esterolytic activity. Study of recombinant Fab fragments and separate light and heavy chains of 9A8 confirmed that the antibody variable domain encodes the catalytic function, whereas neither part of the primary sequence of the Fab exhibited homology with… Expand
Idiotypic mimicry of a catalytic antibody active site.
TLDR
Results indicate idiotypic mimicry of the catalytic antibody's active site, suggesting that the catalytics activity is due to affinity maturation of immunoglobulin genes in response to a specific antigen, namely, the PAS of AChE. Expand
Idiotypic network mimicry and antibody catalysis: lessons for the elicitation of efficient anti-idiotypic protease antibodies.
TLDR
Preliminary results for the production of anti-idiotypic antibodies mimicking subtilisin are presented, which exhibit not only an amidase activity against synthetic substrates, but are also able to cleave a protein, bovine serum albumin (BSA). Expand
Catalytic antibodies: balancing between Dr. Jekyll and Mr. Hyde
TLDR
The conflicting Dr. Jekyll and Mr. Hyde protective and destructive essences of catalytic antibodies should be carefully considered in the development of therapeutic abzyme applications. Expand
Characterization of immune complexes of idiotypic catalytic and anti-idiotypic inhibitory antibodies in plasma of type 1 diabetic subjects.
TLDR
An extensive proteomic investigation on plasma of type 1 diabetic subjects to discover why some of them apparently lacked any measurable proteolytic activity was found enclosed in immune complexes in which Fab/(Fab)(2) displayed a serine-like catalytic activity. Expand
Ontogeny of Proteolytic Immunity
TLDR
Observed chemical activity of immunoglobulin μ and κ/λ subunits expressed on the surface of B cells and in secreted IgM antibodies found in the preimmune repertoire indicate the existence of serine protease-like BCRs and secreted igM Abs as innate immunity components with potential roles in B cell development and Ab effector functions. Expand
Endopeptidase character of monoclonal antibody i41-7 subunits.
TLDR
Both chains of i41-7 mAb could cleave peptide bond of some peptides such as a polypeptide, TP41-1 (TPRGPDRPEGIEEEGGERDRD), as anticipated and the cleaving reaction advanced in accordance with Michaelis-Menten equation. Expand
Anti-idiotypic antibodies: a new approach in prion research
TLDR
The results demonstrate that it is possible to induce a strong anti-idiotypic immune response against the V5B2 monoclonal antibody in both xenogeneic and syngeneic experimental systems and provide a new experimental approach that is applicable to the field of prion diseases. Expand
The antiidiotypic approach to obtaining a proteolytic antibody
TLDR
Construction of catalytic vaccines that are based on such proteases and are capable of target disintegration of proteins responsible for development of certain pathologies could become one of the ways of obtaining drugs of the future. Expand
Prospects for immunotherapeutic proteolytic antibodies.
TLDR
Prospects for development of therapeutic CAbs to the envelope protein gp120 of HIV are described, exploiting the natural tendency of the immune system to synthesize germline-encoded, serine protease-like CAbs. Expand
Investigations into the development of catalytic activity in anti‐acetylcholinesterase idiotypic and anti‐idiotypic antibodies
TLDR
The development of AChE‐like catalytic activity in anti‐AChE Ab1s and Ab2s appears to be the result of a combination of structural complementarity to a substrate‐binding site, charge complementity to a salt bridge, and specific structural peculiarities of the AChe molecule. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 37 REFERENCES
Monoclonal anti-idiotypic antibodies as functional internal images of enzyme active sites: production of a catalytic antibody with a cholinesterase activity.
TLDR
Hydrolysis of acetylthiocholine and related esters of thiocholine by 9A8 follows saturation kinetics and kinetic parameters were determined and exhibits a relaxation of specificity toward both substrates and inhibitors. Expand
Functional mimicry: elicitation of a monoclonal antiidiotypic antibody hydrolizing β‐lactams
  • B. Avalle, D. Thomas, A. Friboulet
  • Chemistry, Medicine
  • FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 1998
TLDR
The relevance of the idiotypic mimicry concept for the generation of catalytic antibodies was further demonstrated by eliciting a third generation antibody (Ab3), which was shown to recognize β‐lactamase. Expand
Anti‐idiotypic antibodies: biological function and structural studies
  • Y. Pan, S. Yuhasz, L. Amzel
  • Chemistry, Medicine
  • FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 1995
TLDR
Structural data at the atomic level have emerged for anti‐idiotypic antibodies from X‐ray diffraction studies and appear to be present as evidenced by the sequence homology between the CDR loops of theAnti‐id and the epitope of the original antigen. Expand
The Immunological Evolution of Catalysis
TLDR
The crystal structure and analysis of somatic and directed active site mutants underscore the role of transition state stabilization in the evolution of this catalytic antibody. Expand
Probing the importance of second sphere residues in an esterolytic antibody by phage display.
TLDR
Libraries derived from the humanized Fab fragment of the antibody 17E8 (h17E8) on filamentous phage were displayed and sorted for binding to an immobilized transition-state analog (TSA), indicating that residues remote from the active site do modulate catalytic activity. Expand
DNA‐specific antiidiotypic antibodies in the sera of patients with autoimmune diseases
TLDR
The stable positive response of autoimmune sera to anti‐topoisomerase monoclonal antibodies has a specific character and is associated with the interaction of the Fab fragment of MAB with the IgG fraction of autoimmune serum. Expand
Dissection of an antibody-catalyzed reaction.
TLDR
Several lines of evidence indicate that the catalytic mechanism of antibody 43C9 involves two key residues: His-L91, which acts as a nucleophile to form the acyl-antibody intermediate, and Arg-L96, which stabilizes the anionic tetrahedral moieties. Expand
Structural basis for amide hydrolysis catalyzed by the 43C9 antibody.
TLDR
The crystallographic structures that are presented here for the single-chain variable fragment of the 43C9 antibody strongly support and extend the structural and mechanistic information previously provided by a three-dimensional computational model, together with extensive biochemical, kinetics, and mutagenesis results. Expand
Structural Convergence in the Active Sites of a Family of Catalytic Antibodies
TLDR
The x-ray structures of three esterase-like catalytic antibodies identified by screening for catalytic activity the entire hybridoma repertoire, elicited in response to a phosphonate transition state analog (TSA) hapten, were analyzed, suggesting that evolution for binding to a single TSA followed by screened for catalysis lead to antibodies with structural convergence. Expand
Phage display of a catalytic antibody to optimize affinity for transition-state analog binding.
TLDR
Phage display methods can be readily used to optimize binding of catalytic antibodies to transition-state analogs, and when used in conjunction with limited screening for catalysis can identify variants with higher catalytic efficiencies. Expand
...
1
2
3
4
...