Enzyme-linked immunospot assays for direct ex vivo measurement of vaccine-induced human humoral immune responses in blood

@article{Saletti2013EnzymelinkedIA,
  title={Enzyme-linked immunospot assays for direct ex vivo measurement of vaccine-induced human humoral immune responses in blood},
  author={G. Saletti and N. Çuburu and J. S. Yang and A. Dey and C. Czerkinsky},
  journal={Nature Protocols},
  year={2013},
  volume={8},
  pages={1073-1087}
}
  • G. Saletti, N. Çuburu, +2 authors C. Czerkinsky
  • Published 2013
  • Biology, Medicine
  • Nature Protocols
  • The enzyme-linked immunospot (ELISPOT) assay was originally developed to enumerate antigen-specific antibody-secreting cells (ASCs), and has subsequently been adapted for various applications, including the detection cytokine-secreting cells. Owing to its exceptionally high sensitivity, the ELISPOT has proven to be especially useful for detecting discrete populations of active cells (e.g., antigen-specific cells). Because of its versatility, the ELISPOT assay is used for a wide range of… CONTINUE READING
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