Enzyme-linked immunosorbent assay for shigella toxin.

  title={Enzyme-linked immunosorbent assay for shigella toxin.},
  author={Arthur Donohue-Rolfe and Michele A. Kelley and Michael Louis Bennish and Gerald T Keusch},
  journal={Journal of clinical microbiology},
  volume={24 1},
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of shigella toxin. For the assay, a mouse monoclonal antibody against the B subunit of the toxin and a rabbit polyclonal antibody against the holotoxin were employed. The monoclonal antibody was used to coat wells of a microtiter plate, and the polyclonal antibody preparation was… CONTINUE READING