Enzyme linked immunosorbent assay for potency testing of vaccines containing Clostridium perfringens type D epsilon-toxoid.

@article{Pfahler1998EnzymeLI,
  title={Enzyme linked immunosorbent assay for potency testing of vaccines containing Clostridium perfringens type D epsilon-toxoid.},
  author={Wolfram Pfahler and E Bisesti and J B Pereyra and Francisco Javier Iribarren},
  journal={Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B},
  year={1998},
  volume={45 3},
  pages={133-9}
}
For ethical, economic and technical reasons in vivo assays need to be replaced in routine laboratory procedures. Based on a method which is already accepted by the British authorities, an indirect ELISA has been developed and evaluated for Clostridium perfringens type D-containing vaccines. Individual and pooled sera of vaccinated rabbits were tested at a single dilution level, the results transferred into IU/ml, and compared with the conventional toxin neutralization test in mice. The ELISA… CONTINUE READING

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