Enzyme-linked immunosorbent assay for leucine and methionine enkephalins.


An enzyme-linked immunosorbent assay for enkephalins was developed by coupling the peptides to a carrier molecule (bovine serum albumin) in order to allow the antibody-antigen reaction to take place in the solid phase. The assay was shown to be highly reproducible. Its sensitivity was 14 nmol/liter for leucine enkephalin and 27 nmol/liter for methionine enkephalin, which is similar to that obtained when the same antibodies were used in radioimmunoassay.

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@article{Zamboni1983EnzymelinkedIA, title={Enzyme-linked immunosorbent assay for leucine and methionine enkephalins.}, author={Giovanni Zamboni and Christine Ann Jones and Janice L. Hughes}, journal={Analytical biochemistry}, year={1983}, volume={130 1}, pages={83-7} }