Enzyme kinetics determined using calorimetry: a general assay for enzyme activity?

  title={Enzyme kinetics determined using calorimetry: a general assay for enzyme activity?},
  author={Matthew J. Todd and J. Garcı́a G{\'o}mez},
  journal={Analytical biochemistry},
  volume={296 2},
  • M. Todd, J. Gómez
  • Published 15 September 2001
  • Biology, Chemistry
  • Analytical biochemistry
Two techniques for determining enzyme kinetic constants using isothermal titration microcalorimetry are presented. The methods are based on the proportionality between the rate of a reaction and the thermal power (heat/time) generated. (i) An enzyme can be titrated with increasing amounts of substrate, while pseudo-first-order conditions are maintained. (ii) Following a single injection, the change in thermal power as substrate is depleted can be continuously monitored. Both methods allow… 

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