Enzyme immunoassays for the estimation of adenosine 3',5' cyclic monophosphate and guanosine 3',5' cyclic monophosphate in biological fluids.

@article{Horton1992EnzymeIF,
  title={Enzyme immunoassays for the estimation of adenosine 3',5' cyclic monophosphate and guanosine 3',5' cyclic monophosphate in biological fluids.},
  author={Julie K. Horton and Ruth C. Martin and Sian Kalinka and Alice Cushing and J P Kitcher and Michael O'Sullivan and P M Baxendale},
  journal={Journal of immunological methods},
  year={1992},
  volume={155 1},
  pages={31-40}
}
Simple, rapid and sensitive competitive enzyme immunoassays for the estimation of adenosine 3',5' cyclic monophosphate (cAMP) and guanosine 3',5' cyclic monophosphate (cGMP) in human plasma and urine are described. Specific antisera to each nucleotide were raised in rabbits by immunization with succinyl cyclic nucleotide--human serum albumin conjugates. For the assay, specific antibodies were incubated with a mixture of succinyl cyclic nucleotide labelled with horseradish peroxidase together… CONTINUE READING

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