Enzyme immunoassays for the determination of ovine LH and FSH.

@article{Peclaris2003EnzymeIF,
  title={Enzyme immunoassays for the determination of ovine LH and FSH.},
  author={G M Peclaris and Athina Pappa and Konstantinos Deligiannis and K. Koutsotolis},
  journal={Reproduction in domestic animals = Zuchthygiene},
  year={2003},
  volume={38 5},
  pages={
          367-72
        }
}
The development of competitive enzyme immunoassays for ovine plasma LH (oLH) and FSH (oFSH) is described. Standards and plasma samples were preincubated with diluted antiserum to oLH or oFSH and the reacted solution (100 micro l per well) was transferred to plates previously coated with oLH or oFSH, respectively. The second antibody used was anti-rabbit IgG horseradish peroxidase. The measuring range was 0.39-50 ng/ml for each hormone and the 50% relative binding sensitivity was 9 ng/ml for oLH… CONTINUE READING

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