Enzyme immunoassay of benzo[a]pyrene conjugated to DNA, RNA and microsomal proteins using a monoclonal antibody.

Abstract

An enzyme immunoassay for the detection of benzo[a]pyrene covalently conjugated to macromolecules has been developed. The monoclonal antibody, raised through in vitro immunization reacted with benzo[a]pyrene metabolites bound to DNA, RNA and proteins. The lower detection limit for the assay was 1 pmol for benzo[a]pyrene bound to DNA or RNA, and 5 pmol when bound to protein.

Cite this paper

@article{Wallin1984EnzymeIO, title={Enzyme immunoassay of benzo[a]pyrene conjugated to DNA, RNA and microsomal proteins using a monoclonal antibody.}, author={H{\aa}kan Wallin and Carl A. K. Borrebaeck and Cristina Glad and Bo Mattiasson and Bengt Jergil}, journal={Cancer letters}, year={1984}, volume={22 2}, pages={163-70} }