Enzyme immunoassay for erythrocyte aldose reductase.

  title={Enzyme immunoassay for erythrocyte aldose reductase.},
  author={Chisa Nishimura and Yoji Hamada and Tetsuya Tachikawa and Tomoko Ishikawa and Tong Gui and J Tsubouchi and Nigishi Hotta and Tsuyoshi Tanimoto and Teizi Urakami},
  journal={Clinical chemistry},
  volume={40 6},
This two-site immunoassay measures erythrocyte aldose reductase by using monoclonal and polyclonal antibodies to recombinant human enzyme. Total incubation time is 2.5 h, and the limit of detection is < 0.05 microgram/L. Analytical recovery tested with blood samples from healthy and diabetic individuals was 101-106%. Average CVs within and between assays were 3.7% and 4.8%, respectively. The enzyme content determined by this system correlated well with the activity of aldose reductase isolated… CONTINUE READING

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