Enzyme dynamics during catalysis measured by NMR spectroscopy.

@article{Kern2005EnzymeDD,
  title={Enzyme dynamics during catalysis measured by NMR spectroscopy.},
  author={Dorothee Kern and Elan Zohar Eisenmesser and Magnus Wolf-Watz},
  journal={Methods in enzymology},
  year={2005},
  volume={394},
  pages={
          507-24
        }
}
Many biological processes, in particular enzyme catalysis, occur in the microsecond to millisecond time regime. While the chemical events and static structural features of enzyme catalysis have been extensively studied, very little is known about dynamic processes of the enzyme during the catalytic cycle. Dynamic NMR methods such as ZZ-exchange, line-shape analysis, Carr-Purcell-Meiboom-Gill (CPMG), and rotating frame spin-lattice relaxation (R(1rho)) experiments are powerful in detecting… CONTINUE READING

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