Enzyme-complemented activatorsorbent assay (ECASA): genetic engineering for enzyme-linked immunosorbent assay-type mercuric ion detection.


The sensor component of bacterial mercury resistance systems is the metalloregulatory protein MerR, which has nanomolar sensitivity and high selectivity for Hg(II). A fusion protein of MerR and the alpha-peptide part of beta-galactosidase (LacZalpha) was constructed by fusing the relevant genes. The protein exhibited both MerR functions and alpha… (More)


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