Enzyme colorimetric assay using unmodified silver nanoparticles.

  title={Enzyme colorimetric assay using unmodified silver nanoparticles.},
  author={Hui Wei and Chaogui Chen and Bingyan Han and Erkang Wang},
  journal={Analytical chemistry},
  volume={80 18},
Colorimetric assay based on the unique surface plasmon resonance properties of metallic nanoparticles has received considerable attention in bioassay due to its simplicity, high sensitivity, and low cost. Most of colorimetric methods previously reported employed gold nanoparticles (GNPs) as sensing elements. In this work, we develop a sensitive, selective, simple, and label-free colorimetric assay using unmodified silver nanoparticle (AgNP) probes to detect enzymatic reactions. Enzymatic… CONTINUE READING
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