Enzyme and phospholipid asymmetry in liver microsomal membranes

  title={Enzyme and phospholipid asymmetry in liver microsomal membranes},
  author={Olle S. Nilsson and Gustav Dallner},
  journal={The Journal of Cell Biology},
  pages={568 - 583}
The transverse distribution of enzyme proteins and phospholipids within microsomal membranes was studied by analyzing membrane composition after treatment with proteases and phospholipases. Upon trypsin treatment of closed microsomal vesicles, NADH- and NADPH-cytochrome c reductases as well as cytochrome b5 were solubilized or inactivated, while cytochrome P-450 was partially inactivated. When microsomes were exposed to a concentration of deoxycholate which makes them permeable to… CONTINUE READING