Enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli. Purification to homogeneity and some properties.

@article{Waygood1980EnzymeIO,
  title={Enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli. Purification to homogeneity and some properties.},
  author={E Bruce Waygood and T Steeves},
  journal={Canadian journal of biochemistry},
  year={1980},
  volume={58 1},
  pages={40-8}
}
Enzyme I of the phosphoenolpyruvate - sugar phosphotransferase system (PTS) has been purified to homogeneity from Escherichia coli. A merodiploid strain P650 which had an extra copy of the gene for enzyme I resulting in a twofold increase in the amount of activity was used. The enzyme is a dimer of 67 000 +/- 5000 molecular weight subunits. At low protein concentration and 4 degrees C the monomer predominates, while at room temperature the dimer predominates. At higher protein concentrations (2… CONTINUE READING