Enzyme I and HPr from Lactobacillus casei: their role in sugar transport, carbon catabolite repression and inducer exclusion.

@article{Viana2000EnzymeIA,
  title={Enzyme I and HPr from Lactobacillus casei: their role in sugar transport, carbon catabolite repression and inducer exclusion.},
  author={Rosa Viana and Vicente Monedero and V. Dossonnet and Christian Vadeboncoeur and Gaspar P{\'e}rez-Mart{\'i}nez and Josef Deutscher},
  journal={Molecular microbiology},
  year={2000},
  volume={36 3},
  pages={570-84}
}
We have cloned and sequenced the Lactobacillus casei ptsH and ptsI genes, which encode enzyme I and HPr, respectively, the general components of the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS). Northern blot analysis revealed that these two genes are organized in a single-transcriptional unit whose expression is partially induced. The PTS plays an important role in sugar transport in L. casei, as was confirmed by constructing enzyme I-deficient L. casei mutants, which were… CONTINUE READING
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