Enzyme-Free Dissociation of Neurospheres by a Microfluidic Chip-Based Method.

  title={Enzyme-Free Dissociation of Neurospheres by a Microfluidic Chip-Based Method.},
  author={Ching-Hui Lin and Hao-Chen Chang and Don-Ching Lee and Ing-Ming Chiu and Chia-Hsien Hsu},
  journal={Methods in molecular biology},
Neurosphere assay is a common and robust method for identification of neural stem/progenitor cells, but obtaining large numbers of live single cells from dissociated neurospheres is difficult using nonenzymatic methods. Here, we present an enzyme-free method for high-efficiency neurosphere dissociation into single cells using microfluidic device technology. This method allows single cell dissociation of DC115 and KT98 cells with high cell viabilities (80-85 %), single-cell yield (91-95 %), and… CONTINUE READING

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