Enzyme Free Cloning for high throughput gene cloning and expression

  title={Enzyme Free Cloning for high throughput gene cloning and expression},
  author={Rob N. de Jong and Mark A. Dani{\"e}ls and Rob Kaptein and Gert E. Folkers},
  journal={Journal of Structural and Functional Genomics},
Structural and functional genomics initiatives significantly improved cloning methods over the past few years. Although recombinational cloning is highly efficient, its costs urged us to search for an alternative high throughput (HTP) cloning method. We implemented a modified Enzyme Free Cloning (EFC) procedure, a PCR-only method that eliminates all variables other than PCR efficiency by circumventing enzymatic treatments. We compared the cloning efficiency of EFC with that of Ligation… CONTINUE READING