Enzyme–Inhibitor Interactions and a Simple, Rapid Method for Determining Inhibition Modality

  title={Enzyme–Inhibitor Interactions and a Simple, Rapid Method for Determining Inhibition Modality},
  author={Shane M. Buker and Paula Ann Boriack-Sjodin and Robert A Copeland},
  journal={SLAS Discovery},
  pages={515 - 522}
Contemporary chemical biology and drug discovery are increasingly focused on the discovery of inhibitory molecules that interact with enzyme targets in specific ways, such as allosteric or orthosteric binding. Hence, there is increasing interest in evaluating hit compounds from high-throughput diversity screening to determine their mode of interaction with the target. In this work, the common inhibition modalities are reviewed and clarified. The impact of substrate concentration, relative to… 

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Response to the Article “Enzyme–Inhibitor Interactions and a Simple, Rapid Method for Determining Inhibition Modality”

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This article was quite disappointed in its one-sided biased endorsement of classical inhibition models and the omission of this point greatly reduces the overall usefulness of a review.

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