Enzymatic synthesis of polymethylated flavonols in Chrysosplenium americanum. I. Partial purification and some properties of S-adenosyl-L-methionine:flavonol 3-, 6-, 7-, and 4'-O-methyltransferases.

@article{DeLuca1985EnzymaticSO,
  title={Enzymatic synthesis of polymethylated flavonols in Chrysosplenium americanum. I. Partial purification and some properties of S-adenosyl-L-methionine:flavonol 3-, 6-, 7-, and 4'-O-methyltransferases.},
  author={Vincenzo De Luca and Ragai K. Ibrahim},
  journal={Archives of biochemistry and biophysics},
  year={1985},
  volume={238 2},
  pages={
          596-605
        }
}
Purification, properties and kinetic mechanism of flavonol 8-O-methyltransferase from Lotus corniculatus L.
A novel O-methyltransferase catalyzing the transfer of the methyl group of S-adenosyl-L-methionine to the 8-hydroxyl group of flavonols was purified about 1200-fold from Lotus flower buds, by
Partial Purification, Kinetic Analysis, and Amino Acid Sequence Information of a Flavonol 3-O-Methyltransferase from Serratula tinctoria1
TLDR
The flavonol 3-O-methyltransferase (3-OMT) was partially purified by ammonium sulfate precipitation and successive chromatography on Superose-12, Mono-Q, and adenosine-agarose affinity columns, resulting in a 194-fold increase of its specific activity.
Biotechnological Production of Dimethoxyflavonoids Using a Fusion Flavonoid O-Methyltransferase Possessing Both 3'- and 7-O-Methyltransferase Activities.
TLDR
Results indicate that dimethoxyflavonoids may be efficiently produced from nonmethylated flavonoid precursors through a one-step biotransformation using the engineered E. coli harboring the SlOMT3/OsNOMT fusion gene.
Novel flavonol 2-oxoglutarate dependent dioxygenase: affinity purification, characterization, and kinetic properties.
TLDR
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Characterization of two cDNA clones which encode O-methyltransferases for the methylation of both flavonoid and phenylpropanoid compounds.
TLDR
The fact that C. americanum accumulates a variety of highly methylated flavonols and exhibits little lignification suggests that these two flavonoid OMT clones have retained their ability to O-methylate phenylpropanoids as well.
Purification and kinetics of phenylpropanoid O-methyltransferase activities from Brassica oleracea
Two phenylpropanoid O-methyltransferase isoforms were purified to homogeneity from young cabbage leaves. They catalyzed the meta-O-methylation of caffeic and 5-hydroxyferulic acids to ferulic and
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References

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Purification and Properties of Flavonol-Ring B Glucosyltransferase from Chrysosplenium americanum.
TLDR
The differential effect on enzyme activity of metal ions, especially cupric ion, and various SH group reagents seemed to indicate the involvement of two active sites in the glucosylation reaction; the site specific for 2' activity being more susceptible than that of the 5' activity.
Purification and properties of a S-adenosylmethionine: isoflavone 4'-O-methyltransferase from cell suspension cultures of Cicer arietinum L.
TLDR
It can be concluded that methylation is the last step in the biosynthesis of 4′-methoxyisoflavones, and follows an Ordered BiBi mechanism with S-adenosylmethionine and S- adenosylhomocysteine as leading reaction partners.
Purification, characterization, and kinetic mechanism of S-adenosyl-L-methionine: vitexin 2"-O-rhamnoside 7-O-methyltransferase of Avena sativa L.
TLDR
An O-methyltransferase catalyzing the transfer of the methyl group of S-adenosyl-L-methionine to the A-ring 7-hydroxyl group of vitexin 2"-O-rhamnoside is isolated from oat primary leaves and a mono-iso Theorell-Chance mechanism is proposed with the nucleotide substrate binding before the flavonoid.
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