Enzymatic synthesis of polymethylated flavonols in Chrysosplenium americanum. I. Partial purification and some properties of S-adenosyl-L-methionine:flavonol 3-, 6-, 7-, and 4'-O-methyltransferases.

@article{DeLuca1985EnzymaticSO,
  title={Enzymatic synthesis of polymethylated flavonols in Chrysosplenium americanum. I. Partial purification and some properties of S-adenosyl-L-methionine:flavonol 3-, 6-, 7-, and 4'-O-methyltransferases.},
  author={Vincenzo De Luca and Ragai K. Ibrahim},
  journal={Archives of biochemistry and biophysics},
  year={1985},
  volume={238 2},
  pages={
          596-605
        }
}
View on PubMed
doi.org
41 Citations
Background Citations
11
Methods Citations
2
Results Citations
3

41 Citations

Citation Type

Citation Type

Enzymatic synthesis of polymethylated flavonols in Chrysosplenium americanum. III. Purification and kinetic analysis of S-adenosyl-L-methionine:3-methylquercetin 7-O-methyltransferase.
Enzymatic synthesis of polymethylated flavonols in Chrysosplenium americanum. II. Substrate interaction and product inhibition studies of flavonol 3-, 6-, and 4'-O-methyltransferases.
Purification, properties and kinetic mechanism of flavonol 8-O-methyltransferase from Lotus corniculatus L.
A novel O-methyltransferase catalyzing the transfer of the methyl group of S-adenosyl-L-methionine to the 8-hydroxyl group of flavonols was purified about 1200-fold from Lotus flower buds, by…
Partial Purification, Kinetic Analysis, and Amino Acid Sequence Information of a Flavonol 3-O-Methyltransferase from Serratula tinctoria1
TLDR
The flavonol 3-O-methyltransferase (3-OMT) was partially purified by ammonium sulfate precipitation and successive chromatography on Superose-12, Mono-Q, and adenosine-agarose affinity columns, resulting in a 194-fold increase of its specific activity.
Biotechnological Production of Dimethoxyflavonoids Using a Fusion Flavonoid O-Methyltransferase Possessing Both 3'- and 7-O-Methyltransferase Activities.
TLDR
Results indicate that dimethoxyflavonoids may be efficiently produced from nonmethylated flavonoid precursors through a one-step biotransformation using the engineered E. coli harboring the SlOMT3/OsNOMT fusion gene.
Novel flavonol 2-oxoglutarate dependent dioxygenase: affinity purification, characterization, and kinetic properties.
TLDR
This is the first reported 2-oxoglutarate-dependent dioxygenase that catalyzes the aromatic hydroxylation of a flavonoid compound and the results of substrate interaction kinetics and product inhibition studies suggest an ordered, sequential reaction mechanism.
Fast protein liquid chromatographic purification and some properties of a partially O-methylated flavonol glucoside 2′-/5′-O-methyltransferase
Characterization of two cDNA clones which encode O-methyltransferases for the methylation of both flavonoid and phenylpropanoid compounds.
TLDR
The fact that C. americanum accumulates a variety of highly methylated flavonols and exhibits little lignification suggests that these two flavonoid OMT clones have retained their ability to O-methylate phenylpropanoids as well.
Characterization of two candidate flavone 8-O-methyltransferases suggests the existence of two potential routes to nevadensin in sweet basil.
Purification and kinetics of phenylpropanoid O-methyltransferase activities from Brassica oleracea
Two phenylpropanoid O-methyltransferase isoforms were purified to homogeneity from young cabbage leaves. They catalyzed the meta-O-methylation of caffeic and 5-hydroxyferulic acids to ferulic and…
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 16 REFERENCES
Purification and properties of an o-dihydricphenol meta-O-methyltransferase from cell suspension cultures of parsley and its relation to flavonoid biosynthesis.
Purification and Properties of Flavonol-Ring B Glucosyltransferase from Chrysosplenium americanum.
TLDR
The differential effect on enzyme activity of metal ions, especially cupric ion, and various SH group reagents seemed to indicate the involvement of two active sites in the glucosylation reaction; the site specific for 2' activity being more susceptible than that of the 5' activity.
Purification and properties of a S-adenosylmethionine: isoflavone 4'-O-methyltransferase from cell suspension cultures of Cicer arietinum L.
TLDR
It can be concluded that methylation is the last step in the biosynthesis of 4′-methoxyisoflavones, and follows an Ordered BiBi mechanism with S-adenosylmethionine and S- adenosylhomocysteine as leading reaction partners.
Characterization of three distinct flavonol O-methyltransferases from Chrysosplenium americanum
Purification, characterization, and kinetic mechanism of S-adenosyl-L-methionine: vitexin 2"-O-rhamnoside 7-O-methyltransferase of Avena sativa L.
TLDR
An O-methyltransferase catalyzing the transfer of the methyl group of S-adenosyl-L-methionine to the A-ring 7-hydroxyl group of vitexin 2"-O-rhamnoside is isolated from oat primary leaves and a mono-iso Theorell-Chance mechanism is proposed with the nucleotide substrate binding before the flavonoid.
Biosynthesis of a retrochalcone, echinatin: Involvement of O-methyltransferase to licodione☆
O-Methylation of flavonoid substrates by a partially purified enzyme from soybean cell suspension cultures.
Two forms of O-methyltransferase in tobacco cell suspension culture
Untersuchungen zum Vorkommen zweier O-Methyltransferasen in Tulipa-Antheren
O-methylation of flavonoids by cell-free extracts of calamondin orange
...
1
2
...