Enzymatic synthesis of S-adenosyl-L-methionine from L-methionine and ATP.

@article{Gross1983EnzymaticSO,
  title={Enzymatic synthesis of S-adenosyl-L-methionine from L-methionine and ATP.},
  author={Adrian Gross and Shimona Geresh and George M Whitesides},
  journal={Applied biochemistry and biotechnology},
  year={1983},
  volume={8 5},
  pages={415-22}
}
S-Adenosyl-L-methionine (SAM) has been synthesized on 7-mmol scale from L-methionine and ATP. The ATP was generated in situ from AMP, and the synthesis of SAM was catalyzed by the enzyme ATP:L-methionine S-adenosyltransferase (EC 2.5.1.6). Although substantial effort was required to obtain even small quantities of enzyme, it has good stability once isolated and immobilized. The SAM produced by this procedure contains 89% of the (-) diasterioisomer and 11% of the (+) diasterioisomer.