Enzymatic sialylation of N-linked oligosaccharides using an alpha-(2,3)-specific trans-sialidase from Trypanosoma cruzi: structural identification using a three-dimensional elution mapping technique.

@article{Takahashi1995EnzymaticSO,
  title={Enzymatic sialylation of N-linked oligosaccharides using an alpha-(2,3)-specific trans-sialidase from Trypanosoma cruzi: structural identification using a three-dimensional elution mapping technique.},
  author={Noriko Takahashi and Kyung Bok Lee and Hiroaki Nakagawa and Yoshinori Tsukamoto and Yoshiya Kawamura and Yu-Teh Li and Yuan Chuan Lee},
  journal={Analytical biochemistry},
  year={1995},
  volume={230 2},
  pages={333-42}
}
alpha-(2,3)-Sialylated biantennary and triantennary oligosaccharides were enzymatically prepared from pyridyl-2-amino-oligosaccharides with terminal Gal residues, using an alpha-(2,3)-specific trans-sialidase from Trypanosoma cruzi (Lee, K. B., and Lee, Y. C. (1994) Anal. Biochem. 216, 358-364). From the pyridyl-2-amino-derivatives of neutral and alpha-(2,6)-monosialylated biantennary oligosaccharides from human fibrinogen, 5 different sialyl biantennary oligosaccharides were obtained. From two… CONTINUE READING