Enzymatic remodelling of the carbohydrate surface of a xenogenic cell substantially reduces human antibody binding and complement-mediated cytolysis

@article{Sandrin1995EnzymaticRO,
  title={Enzymatic remodelling of the carbohydrate surface of a xenogenic cell substantially reduces human antibody binding and complement-mediated cytolysis},
  author={Mauro Sergio Sandrin and William L. Fodor and Effie Mouthtouris and Narin Osman and Shlomo J Cohney and Scott A. Rollins and Edward R. Guilmette and Eva Setter and Stephen P. Squinto and Ian F.C. Mckenzie},
  journal={Nature Medicine},
  year={1995},
  volume={1},
  pages={1261-1267}
}
The major obstacle to successful discordant xenotransplantation is the phenomenon of hyperacute rejection (HAR). In the pig-to-primate discordant transplant setting, HAR results from the deposition of high-titre anti-α-galactosyl antibodies and complement activation leading to endothelial cell destruction and rapid graft failure. To overcome HAR, we developed an enzymatic carbohydrate remodelling strategy designed to replace expression of the Galα-1,3-Gal xenoepitope on the surface of porcine… CONTINUE READING