Enzymatic production of cyclodextrins

@article{Biwer2002EnzymaticPO,
  title={Enzymatic production of cyclodextrins},
  author={Arno P. Biwer and Garo Antranikian and Elmar Heinzle},
  journal={Applied Microbiology and Biotechnology},
  year={2002},
  volume={59},
  pages={609-617}
}
Abstract. Cyclodextrins (CD) are enzymatically modified starches with a wide range of applications in food, pharmaceutical and chemical industries, agriculture and environmental engineering. They are produced from starch via enzymatic conversion using cyclodextrin glycosyl transferases (CGTases) and partly α-amylases. Due to its low solubility in water, separation and purification of β-CD is relatively easy compared to α- and γ-CD. In recent years more economic processes for γ-CD and especially… 
Engineering of cyclodextrin glucanotransferases and the impact for biotechnological applications
TLDR
This review focuses on the strategies employed in obtaining CGTases with new or enhanced enzymatic capabilities by searching for new enzymes and improving existing enzyme activities via protein engineering.
γ-Cyclodextrin: a review on enzymatic production and applications
TLDR
Compared with α- and β-cyclodextrins, γ- cyclodextrin has a larger internal cavity, higher water solubility, and more bioavailability, so it has wider applications in many industries, especially in the food and pharmaceutical industries.
Gamma-Cyclodextrin Production Using Cyclodextrin Glycosyltransferase from Bacillus clarkii 7364
TLDR
The approach described here can be easily applied to an enzymatic process for the production of γ-CD on an industrial scale, and such high selectivity, at high conversions, is especially attractive from a commercial perspective.
The effect of cyclodextrins on the ethanol tolerance of microorganisms suggests potential application
TLDR
It is shown that the presence of CDs may increase the ethanol tolerance of microorganisms and suggests a potential application for the co-production of CDs and ethanol.
Characterization of α-Cyclodextrin Glucanotransferase from Bacillus licheniformis
TLDR
Characterization of α-CGTase on the effect of operating conditions was investigated to improve the production of cyclodextrin (CD) by using the optimal conditions which in turn may be beneficial for the industrial purpose.
Enhancing the cyclodextrin production by synchronous utilization of isoamylase and α-CGTase
TLDR
This is the first report of synchronous bioconversion of CD by both α-CGTase and isoamylase, and represents the highest efficiency of CD production reported so far.
EFFECT OF REACTION CONDITIONS ON THE SYNTHESIS OF CYCLODEXTRIN (CD) BY USING IMMOBILIZED ENZYME
TLDR
The immobilized enzyme exhibited a 1.3-3-fold increase in CD production compared to the free enzyme, and the hollow fiber membrane is suitable to be used as a support for enzyme immobilization with the high production of CD.
Cyclodextrin glucanotransferase: from gene to applications
TLDR
The biological role of the enzyme is proposed based on the genetic and enzymatic analyses and methods to enhance the production of active CGTase by bacteria are compared.
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Production of β-cyclodextrin (CD) with high-dextrose equivalent (DE) starch hydrolysates by simultaneous fermentation and cyclization (SFC) gives higher yields than using only the enzyme CGTase,
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TLDR
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TLDR
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  • Chemistry, Medicine
    Biotechnology and applied biochemistry
  • 1997
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Formation of cyclodextrin (CD) from soluble starch by Bacillus macerans CGTase (cyclodextrin glucanotransferase) was studied experimentally in both systems with and without the addition of ethanol.
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