Enzymatic methylation of in vitro synthesized apocytochrome c enhances its transport into mitochondria.

@article{Park1987EnzymaticMO,
  title={Enzymatic methylation of in vitro synthesized apocytochrome c enhances its transport into mitochondria.},
  author={Kyong Soo Park and B F Frost and Michael Tuck and Ling Ling Ho and Sunkyu Kim and Woon Ki Paik},
  journal={The Journal of biological chemistry},
  year={1987},
  volume={262 30},
  pages={14702-8}
}
The gene for iso-1-cytochrome c from Saccharomyces cerevisiae was recloned into a pSP65 vector containing an active bacteriophage SP promoter. The iso-1-cytochrome c gene was cloned as an 856-base pair XhoI-HindIII fragment. When the resulting plasmid was digested at the HindIII site 279 bases downstream from the termination codon of the gene and transcribed in vitro using SP6 RNA polymerase, full length transcripts were produced. The SP6 iso-1-cytochrome c mRNA was translated using a rabbit… CONTINUE READING

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