Corpus ID: 8369204

Enzymatic methylation of in vitro synthesized apocytochrome c enhances its transport into mitochondria.

@article{Park1987EnzymaticMO,
  title={Enzymatic methylation of in vitro synthesized apocytochrome c enhances its transport into mitochondria.},
  author={K. Park and B. Frost and M. Tuck and L. Ho and S. Kim and W. Paik},
  journal={The Journal of biological chemistry},
  year={1987},
  volume={262 30},
  pages={
          14702-8
        }
}
  • K. Park, B. Frost, +3 authors W. Paik
  • Published 1987
  • Biology, Medicine
  • The Journal of biological chemistry
  • The gene for iso-1-cytochrome c from Saccharomyces cerevisiae was recloned into a pSP65 vector containing an active bacteriophage SP promoter. The iso-1-cytochrome c gene was cloned as an 856-base pair XhoI-HindIII fragment. When the resulting plasmid was digested at the HindIII site 279 bases downstream from the termination codon of the gene and transcribed in vitro using SP6 RNA polymerase, full length transcripts were produced. The SP6 iso-1-cytochrome c mRNA was translated using a rabbit… CONTINUE READING
    In vivo and in vitro arginine methylation of RNA-binding proteins.
    • 294
    • PDF

    References

    Publications referenced by this paper.
    SHOWING 1-2 OF 2 REFERENCES
    U
    • 24,248
    • PDF
    The Enzymology of Posttranslational Modification of Proteins (Freedman
    • 1985