Enzymatic in vitro synthesis of globin genes

  title={Enzymatic in vitro synthesis of globin genes},
  author={Argiris Efstratiadis and Fotis C. Kafatos and Allan M. Maxam and Tom Maniatis},
Quantitative synthesis of full-length globin genes: dependence on template.
Human globin messenger RNA: importance of cloning for structural analysis.
The sequence of most of the human beta globin messenger RNA and large sections of the alpha globin Messenger RNA has been determined and several colonies transformed by these molecules have been shown to hybridize with labeled human globin complementary RNA.
Second-strand cDNA synthesis: classical method.
  • U. Gubler
  • Chemistry, Biology
    Methods in enzymology
  • 1987


Enzymatic multiplication of a chemically synthesized DNA fragment
A synthetic DNA fragment of 19 residues was enlarged by the enzymatic addition of deoxyadenylate residues to its 3'-end with calf thymus terminal deoxynucleotidyl transferase to allow accumulation of the desired strand.
Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase.
A new purification procedure for Escherichia coli DNA polymerase yields about 10 mg of homogeneous enzyme per kg of cell paste, with exonuclease III as a by-product, thus minimizing the possibility of enzyme-associated nucleotide material in stoichiometric amounts.
Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.
We describe the use of polyacrylamide gel electrophoresis to estimate chain lengths of double- and single-stranded DNA molecules in the size range 20-1000 base pairs (or nucleotides). Double-stranded
Covalent Attachment of DNA to Agarose
The DNA-Sepharose is stable to high temperature, prolonged storage, and in the case of single-stranded DNA, can be washed with NaOH to destroy nuclease activity and to release any digested oligon nucleotides or mononucleotides.
Purification of Avian Myeloblastosis Virus DNA Polymerase by Affinity Chromatography on Polycytidylate-Agarose
Results suggest that polyribonucleotide columns may provide a high-yield, rapid method for the purification of oncornaviral DNA polymerase.