Enzymatic hydrolysis of esterified diarrhetic shellfish poisoning toxins and pectenotoxins

@article{Doucet2007EnzymaticHO,
  title={Enzymatic hydrolysis of esterified diarrhetic shellfish poisoning toxins and pectenotoxins},
  author={E. Doucet and Neil N Ross and M. Quilliam},
  journal={Analytical and Bioanalytical Chemistry},
  year={2007},
  volume={389},
  pages={335-342}
}
Okadaic acid (OA) and dinophysistoxins-1 and -2 (DTX1, DTX2), the toxins responsible for incidents of diarrhetic shellfish poisoning (DSP), can occur as complex mixtures of ester derivatives in both plankton and shellfish. Alkaline hydrolysis is usually employed to release parent OA/DTX toxins, and analyses are conducted before and after hydrolysis to determine the concentrations of nonesterified and esterified toxins. Recent research has shown that other toxins, including pectenotoxins and… Expand
Accumulation and esterification of diarrhetic shellfish toxins from the aqueous phase in laboratory-exposed mussels.
TLDR
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TLDR
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TLDR
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LC-MS/MS analysis of diarrhetic shellfish poisoning (DSP) toxins, okadaic acid and dinophysistoxin analogues, and other lipophilic toxins.
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  • Chemistry, Medicine
  • Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
  • 2011
TLDR
Electrospray ionization (ESI) liquid chromatography-mass spectrometry (LC-MS) is a very powerful tool for the detection, identification and quantification of DSP and other lipophilic toxins. Expand
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References

SHOWING 1-10 OF 37 REFERENCES
Detection of okadaic acid esters in the hexane extracts of Spanish mussels.
TLDR
The inclusion of a hydrolytic step of the hexane extract in the general procedure is suggested in order to monitor the contribution of non-polar diarrhoetic shellfish poisons (DSPs) to the total DSP shellfish toxicity. Expand
Biological Activities of Semisynthetic Analogs of Dinophysistoxin-3, the Major Diarrhetic Shellfish Toxin
Dinophysistoxin-3 (DTX3, 3), the principal toxin of scallops implicated in diarrhetic shellfish poisoning, is a mixture of 7-O-acyl esters of 35-methylokadaic acid. To investigate the effects ofExpand
Enteropathogenicity of Diarrhetic Shellfish Toxins in Intestinal Models
TLDR
It is concluded that DST shows enterotoxic activities in these animal models and that okadaic acid, DTX-1 andDTX-3 are diarrheagenic. Expand
Accumulation and transformation of DSP toxins in mussels Mytilus galloprovincialis during a toxic episode caused by Dinophysis acuminata.
TLDR
Only in cases where the intoxication period is very long, can the formed acyl-derivatives be important, because they seem to accumulate for a long time in the mussels, as suggested by the low hydrolysis and depuration rates estimated from model fitting. Expand
Analysis of diarrhetic shellfish poisoning toxins in shellfish tissue by liquid chromatography with fluorometric and mass spectrometric detection.
  • M. Quilliam
  • Chemistry, Medicine
  • Journal of AOAC International
  • 1995
TLDR
Improved methods based on liquid chromatography combined with mass spectrometry and fluorometric detection of anthryldiazomethane (ADAM) derivatives were improved upon to achieve a high degree of accuracy and precision for the determination of DSP toxins in a new mussel tissue reference material (MUS-2). Expand
Diarrhetic shellfish poisoning by okadaic acid esters from Brown crabs (Cancer pagurus) in Norway.
TLDR
Esterified OA constituted more than 90% of total identified DSP toxins in crabs, indicating that not only esterified toxin from mussels was accumulated, but also that appreciable transfer of OA to OA-esters occurred in the crabs. Expand
Identification of fatty acid esters of pectenotoxin-2 seco acid in blue mussels (Mytilus edulis) from Ireland.
TLDR
The most abundant fatty acid esters in the fractionated sample were, in order, the 16:0, 22:6, 14: 0, 16:1, 18:4, and 20:5 fatty acids, although a wide array of other PTX-2 seco acid fatty acids were also present at low levels. Expand
Discovery of okadaic acid esters in the toxic dinoflagellate Dinophysis acuta from New Zealand using liquid chromatography/tandem mass spectrometry.
TLDR
Using various liquid chromatography/electrospray ionization tandem mass spectrometry techniques, the first report identifying the presence of OA esters in Dinophysis species is identified. Expand
Pectenotoxin-2 seco acid: a toxin converted from pectenotoxin-2 by the New Zealand Greenshell mussel, Perna canaliculus.
TLDR
Findings indicate that PTX2SA and 7-epi-PTX 2SA are not artifact toxins resulting from hydrolysis of PTX1, but arise from the conversion of PTx2 by mussel tissues. Expand
Discovery of fatty acid ester metabolites of spirolide toxins in mussels from Norway using liquid chromatography/tandem mass spectrometry.
TLDR
Analyses performed with liquid chromatography/tandem mass spectrometry (LC/MS/MS) have revealed a complex mixture of esters of 20-methyl spirolide G in the contaminated mussels. Expand
...
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